Analysis Note

ControlAdult mouse brain tissue

Application

Anti-Synaptophysin Antibody, clone SY38, Alexa Fluor 488 Conjugate detects level of Synaptophysin & has been published & validated for use in IH.

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. The localization and ubiquitous expression of this protein in neuronal tissue makes it an excellent marker for neurons and neuroendocrine tumors. The gene for this protein is located on the X chromosome (Xp11.23-p11.22). The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.

Immunogen

Vesicular fraction of bovine brain

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Uncongugated antibody (MAB5258) is shown to work on Rat.

Physical form

Protein G purified

Purified mouse monoclonal IgG1κ conjugated to Alexa Fluor^® 488 in buffer containing 15 mg/ml BSA with 0.1% sodium azide.

Quality

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Synaptophysin in adult mouse brain tissue.

Specificity

This antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. It also reacts with vesicles of adrenal medulla and islet cells.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Cat. No. MAB5258) has an observed molecular weight at ~38 kDa